Home Biology Biotechnology Principles and Processes NCERT After completing the transformation experime…
Biology Biotechnology Principles and Processes NCERT Single Correct MCQ
Published on: August 13, 2026

After completing the transformation experiment involving the coding sequence of enzyme a-galactosidase, the recombinant colonies should

A
Give blue colour
B
Not give blue colour &
C
Have active a -galactosidase
D
Both

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Text Solution

Verified by Experts
The correct answer is:
B

In insertional inactivation, the foreign DNA is inserted into the α-galactosidase (lacZ) coding sequence, disrupting enzyme production.

• Recombinant colonies → enzyme inactive → white colonies (no blue colour).

• Non-recombinant colonies → enzyme active → blue colonies.

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